Ocean Rider Seahorse Farm and Tours | Kona Hawaii › Forums › Seahorse Life and Care › Erectus respiration normal; Reidi respiration high › Re:Erectus respiration normal; Reidi respiration h
It sounds like you have an outstanding system for seahorses, sir. Your tank has excellent size of water volume, the sump and the protein skimmer are big pluses, and you have good circulation and water movement with the aid of the powerheads to eliminate dead spots. With the exception of the nitrates, all of the aquarium parameters you listed are excellent.
I know of no pathogens or parasites that are specific to Hippocampus reidi and would not also affect the H. erectus in your seahorse tank.
If you read through the discussion thread on the first page of this forum titled "mild huffing going on…" you’ll see a good summary of the primary factors that affect the respiration rate of seahorses in the aquarium. As you will see if you go over the posts in that discussion thread, one of those factors is high nitrate levels. Ideally, we like to see the nitrate levels remain below 10 ppm, and it’s important for the seahorse keeper to keep the nitrates below 20 if at all possible because high levels of nitrogenous wastes can have an adverse affect on the breathing of the seahorses.
Exposure to moderate levels of ammonia and nitrite, or high levels of nitrates, can change the normal hemoglobin in the seahorse’s blood stream to a form (i.e., methhemoglobin) that is no longer able to transport oxygen. The higher the levels of these wastes and the longer the exposure, the greater the proportion of normal hemoglobin that will be converted to the dysfunctional methhemoglobin molecule instead. If this becomes severe enough, it will leave the affected seahorses starved for oxygen, which makes them very weak and fatigued. As a result, the affected seahorses may detach themselves from their hitching posts periodically and rest on the bottom, unable to exert themselves in their weakened condition. As you can imagine, being deprived of oxygen really wipes them out in terms of loss of energy and stamina. And it also results in respiratory distress, and rapid, labored breathing as they try to oxygenate themselves and compensate for the scarcity of normal hemoglobin.
In your case, Tom, the nitrate levels are marginally high (20 ppm), which is not enough to drain the seahorses of their energy and strength, but may be high enough to affect the respiration rate of sensitive individuals. Some seahorse species, such as Hippocampus barbouri and H. reidi, are more sensitive to elevated nitrate levels than others. Hippocampus erectus is a little more tolerant of high nitrate levels, which may be one reason why your H. erectus are still breathing normally but your H. reidi have begun to exhibit rapid respirations.
One of the properties of methylene blue is that it can reverse this process and convert the methhemoglobin in the red blood cells back into normal hemoglobin, which can then pick up and transport oxygen again as usual. That’s why it is so helpful in relieving shipping stress and treating ammonia exposure and nitrite poisoning. For this reason, you may want to pick up some methylene blue at your local fish store and keep it on hand in case it is ever needed (the Kordon brand of methylene blue is best, in my opinion). So keep a close eye on breathing of your H. reidi, especially compared to the H. erectus that are breathing normally, and be prepared to give them a quick dip in methylene blue as described below.
The usual criteria for determining whether or not methylene blue is needed to help seahorses recover from exposure to high levels of nitrogenous wastes (e.g., ammonia, nitrite, or nitrate) is their respiration. If the seahorse has labored breathing — huffing or rapid respiration — then methylene blue is called for. Likewise, if the seahorse is experiencing convulsions or it’s behavior otherwise indicates it is suffering from more than temporary disorientation and loss of equilibrium, such as lying prostrate on the bottom, unable to right itself again, it may benefit from methylene blue to assist its recovery.
Here are the instructions for treating seahorses with methylene blue, Tom:
Commonly known as "meth blue" or simply "blue," this is a wonderful medication for reversing the toxic effects of ammonia and nitrite poisoning (commonly known as "new tank syndrome"). Since hospital tanks are usually without biological filtration, and ammonia and nitrite can thus build up rapidly (especially if you are not doing water changes during the treatment period), it’s a good idea to add methylene blue to your hospital ward when treating sick fish.
Methylene blue also transports oxygen and aids breathing. It facilitates oxygen transport, helping fish breathe more easily by converting methemoglobin to hemoglobin — the normal oxygen carrying component of fish blood, thus allowing more oxygen to be carried through the bloodstream. This makes it very useful for treating gill infections, low oxygen levels, or anytime your seahorses are breathing rapidly and experiencing respiratory distress. It is the drug of choice for treating hypoxic emergencies of any kind with your fish.
In addition, methylene blue treats fungus and some bacteria and protozoans. Methylene blue is effective in preventing fungal infections, and it has antiprotozoal and antibacterial properties as well, by virtue of its ability to bind with cytoplasmic structures within the cell and interfere with oxidation-reduction processes. A "must" for your fish-room medicine cabinet. However, be aware that it is not safe to combine methylene blue with some antibiotics, so check your medication labels closely for any possible problems before doing so.
If you can obtain the Kordon brand of Methylene Blue (available at most well-stocked local fish stores), the instructions for administering it as a very brief, concentrated dip are as follows:
For use as a dip for treatment of fungus or external parasitic protozoans and cyanide poisoning:
(a) Prepare a nonmetallic container of sufficient size to contain the fish to be treated by adding water similar to the original aquarium.
(b) Add 5 teaspoons (24.65 ml) per 3 gallons of water. This produces a concentration of 50 ppm. It is not recommended that the concentration be increased beyond 50 ppm.
(c) Place fishes to be treated in this solution for no longer than 10 seconds.
(d) Return fish to original aquarium.
When you administer such a dip, hold the seahorse in your hand throughout the procedure and time it closely so that the dip does not exceed 10 seconds.
And here are Kordon’s instructions for administering the methylene blue in a hospital tank if longer-term treatment seems appropriate to reverse more severe cases of nitrite poisoning and ammonia toxicity or exposure to high-level of nitrates:
As an aid in reversal of nitrite (NO2-) or cyanide (CN-) poisoning of marine and freshwater aquarium fishes:
(a) Remove carbon filter and continue to operate with mechanical filter media throughout the treatment period.
(b) Add 1 teaspoon of 2.303% Methylene Blue per 10 gallons of water. This produces a concentration of 3 ppm. Continue the treatment for 3 to 5 days.
(c) Make a water change as noted and replace the filter carbon at the conclusion of the treatment.
See the following link for more information on treating with Kordon’s Methylene Blue:
Click here: KPD-28 Methylene Blue
If you obtained a brand of methylene blue other than Kordon, just follow the instructions the medication comes with. Remember that methylene blue will have an adverse impact on the beneficial bacteria that carry out the nitrogen cycle, so don’t use it in your main tank — rather, use the methylene blue as a quick dip or for treating the seahorses for a prolonged period in your hospital tank.
Here’s what I recommend you concentrate on for the time being, Tom:
1) Reduce the water temperature in your main tank if it is higher than 75°F to increase the ump amount of dissolved oxygen the water can hold.
2) Increase the surface agitation in the aquarium to promote better oxygenation and facilitate efficient gas exchange at the air/water interface.
3) Observe the breathing rate of your seahorses and be prepared to give them a quick dip in the methylene blue if necessary.
4) Reduce the nitrate levels in your aquarium as much as possible.
Here’s a good discussion on controlling nitrates that you may also find helpful in that regard, sir:
Nitrates in the Aquarium: Where They Come from and How to Control Them
In and of themselves, nitrates are relatively harmless and midrange levels are nothing to be too alarmed about. Normally a nitrate reading of 20 ppm is not a cause for concern with seahorses. Ideally, though, we’d like to keep them under 20 ppm at all times and below 10 ppm, it possible, and if your nitrates are running on the high side and you have a problem with hair algae or your seahorse are showing signs of respiratory distress, you need to try to reduce them as much as possible. In case you haven’t already seen it, I am going to provide you with some information on nitrification and denitrification that explains where nitrates come from and then offer you some suggestions on how to reduce them.
The amount of nitrate that accumulates in your aquarium is related to how much nitrification and denitrification your system provides. Nitrification is the process by which aerobic (oxygen loving) nitrifying bacteria break down toxic ammonia to relative harmless nitrate in a series of steps. Nitrification thus ultimately causes nitrate to build up in an aquarium. Denitrification is the process by which anaerobic (oxygen hating) denitrifying bacteria then convert nitrate into completely harmless nitrogen (N2), which eventually leaves the aquarium. Denitrification thus removes nitrate from your system. This entire process is known as the nitrogen cycle.
Cycling your aquarium simply means to build up a healthy population of beneficial bacteria in your tank that can carry out the nitrogen cycle and breakdown your fishes’ waste products. Ammonia (NH3), nitrite (NO2), and nitrate (NO3) are all nitrogenous (nitrogen containing) wastes. All living aquarium animals whether they be fish or invertebrates excrete these wastes, and they are also produced by the decay of protein-containing organic matter (uneaten food, detritus, dead fish or inverts, etc.). The nitrogen cycle breaks down these wastes in a series of steps into nitrogen gas (N2) which leaves the aquarium as bubbles.
The nitrogen cycle begins with ammonia, which is highly poisonous. In the first step of the cycle, Nitrosomonas bacteria reduce ammonia to nitrite, which is also very toxic, but slightly less so. In the second step of the nitrogen cycle, Nitrobacter bacteria convert the nitrite to nitrate, which is relatively harmless but can become harmful when it accumulates in high enough levels. In the third and final step of the cycle, denitrifying bacteria then convert the nitrate into completely harmless N2, which of course bubbles out of the tank as nitrogen gas. In this way, thanks to the nitrogen cycle, dangerous wastes are converted into progressively less harmful compounds and finally removed from the aquarium altogether.
When we set up a new aquarium, and wait for it to cycle, we are simply allowing a big enough population of these different types of bacteria to build up in the biofilter to break down all of the wastes that will be produced when the aquarium is stocked. If we don’t wait long enough for the cycle to complete itself and the biofiltration to become fully established, and hastily add too many specimens to a new aquarium too soon, they will die from ammonia poisoning or nitrite toxicity. This is such a common mistake among us impatient aquarists, that when fish get sick and/or die from ammonia/ntrite poisoning, it is commonly called the "new tank syndrome."
When your aquarium has completely cycled, the ammonia levels will stay at zero because, now that your biofilter is fully established, there is a large enough population of aerobic (oxygen loving) nitrifying Nitrosomonas bacteria to reduce all of the ammonia to nitrite as fast as the ammonia is being produced. The nitrite levels will likewise stay at zero because there is also a large enough population of aerobic (oxygen loving) nitrifying Nitrobacter bacteria to convert all of the nitrite to nitrate as fast as the nitrite is being produced.
The nitrate levels ordinarily continue to build up, however, because there are simply not enough anaerobic (oxygen hating) denitrifying bacteria to convert all of the nitrate that’s being produced into nitrogen (N2). Since nitrates are being produced faster than they can be transformed to nitrogen, the excess nitrates accumulate steadily in your aquarium.
That’s perfectly normal, since the denitrifying bacteria that carry out that final step, the conversion of nitrate (NO3) to nitrogen (N2), are anaerobes that can only exist in the absence of oxygen. For our aquariums to support life, and for the fish and invertebrates to breathe and survive, our tanks must be well aerated and well circulated so that there’s plenty of dissolved oxygen in the water at all times. That means there are normally very few areas in our aquariums where anaerobic denitrifying bacteria can survive, limiting their population accordingly (which is generally good, since some anaerobes produce deadly hydrogen sulfide gas during the decay of organic matter and would poison our tanks if allowed to proliferate).
Consequently, most aquariums lack a sufficient population of anaerobic denitrifying bacteria to complete the nitrogen cycle and convert nitrate to nitrogen as fast as the nitrates are being produced. The only way to keep the nitrates from building up to harmful levels in such setups is with regular water changes and by harvesting Caulerpa or other macroalgae periodically after it has utilized nitrates for growth. Overcrowding, overfeeding, or under filtration exacerbate the problem by resulting in more nitrates being produced and more frequent water changes being required to control the nitrate levels.
Live rock helps because the oxygen-poor interior of the rock allows anaerobic denitrifying bacteria to grow and break down nitrates. A deep live sand bed (DLSB) also helps because anaerobic denitrifying bacteria can flourish and break down nitrates at a certain depth below the sand where oxygenated water no longer penetrates, but a DLSB can sometimes be difficult to set up and manage properly if you’re inexperienced with live sand. Both live rock and deep live sand beds give aquaria denitrification ability — the ability to complete the cycle and convert nitrate to harmless nitrogen. Ordinarily, about 1-2 pounds of live rock per gallon is recommended – that amount of LR will provide your aquarium with all of the biofiltration you need, as well as adequate denitrification ability. You will then keep nitrates at harmless levels by performing regular water changes, harvesting Caulerpa macroalgae periodically, and good aquarium management.
So nitrate is simply the end product of the process of nitrification, formed during the Nitrogen Cycle by the oxidation of nitrite by aerobic bacteria. Nitrates always tend to build up in a system over time, sometimes in sneaky ways you wouldn’t expect. For example, here is an article from Thiel Aqua Tech that discusses some of the hidden ways nitrate can enter your system:
Click here: No nitrate, removal nitrate, denitrating, denitration
One of the sneaky or hidden ways phosphates, nitrates, silicates and other undesirable compounds can enter our aquariums is through the tap water reuse for water changes or topping off our tanks. If the water quality in your town is not what it should be, you may want to consider buying reverse osmosis/deinonized water (RO/DI) for your water changes. Most well-stocked pet shops that handle marine fish sell RO/DI water as a service for their customers for between 25 and 50 cents a gallon. If your LFS does not, WalMart sell RO/DI water by the gallon for around 60 cents, and you should be able to find a Wal-Mart nearby.
Natural seawater is another good option for water changes. Like RO/DI water, natural seawater can be purchased at fish stores for around $1.00 a gallon, depending on where you live. It sounds expensive, but when you consider the alternative — paying for artificial salt mix and RO/DI water and mixing your own saltwater — then natural seawater is not a bad bargain at all. It has unsurpassed water quality and seahorses thrive in it.
You should also be aware that freshly mixed saltwater can have residual levels of ammonia, but if you aerate the newly mixed saltwater for 24-48 before you perform the water changes, the ammonia will be dissipated.
Good ways to reduce nitrates in your aquarium include adding more live rock, installing a deep live sand bed (preferably in a sump), installing a protein skimmer on your tank if your not already using one, and growing and harvesting fast-growing macroalgae such as Caulerpa.
Protein skimmers help reduce nitrates by removing dissolved organics from the water before they can enter the nitrogen cycle. The majority of the undesirable metabolites, organic wastes and excess nutrients that accumulate in our aquariums and degrade water quality are "surface-active," meaning they are attracted to and collect near the surface of a gas-liquid interface. Skimmers take advantage of this fact by using a column of very fine air bubbles mixed with aquarium water to trap dissolved organics and remove them from our systems. This air-water mixture is lighter than the surrounding aquarium and rises up the column of the skimmer until the foam eventually spills into a special collection cup atop the skimmer, which can be removed and emptied as needed. Proteins and other organic molecules, waste products, uneaten food and excess nutrients, and a host of other undesirable compounds stick to the surface of the bubbles and are carried away along with the foam and removed from the aquarium. As a result of this process, these purification devices are typically known as foam separators, foam fractionators, air-strippers, or simply protein skimmers.
In my experience, nothing improves water quality like a good protein skimmer. They provides many benefits for a seahorse setup, including efficient nutrient export, reducing the effective bioload, and increasing both the Redox potential and dissolved oxygen levels in the water. They do a tremendous job of removing excess organics from the aquarium, including phenols, albumin, dissolved organic acids, and chromophoric (color causing) compounds. Their ability to remove dissolved wastes BEFORE they have a chance to break down and degrade water quality makes them indispensable for controlling nuisance algae. A good protein skimmer is an invaluable piece of equipment for keeping your nitrates low and your water quality high when feeding a whole herd of these sloppy eaters in a closed-system aquarium.
I also like the use of macroalgaes for controlling nitrate and nuisance algae. Macroalgae use nitrate for growth just like plant fertilizer and pruning the macros regularly is a good way to export nitrate from your system. However, if the macros die in your system, they’ll release the nitrate they’ve consumed back into the aquarium. Fast-growing Caulerpa needs to be thinned out regularly to prevent vegetative events and avoid this from happening, as discussed below:
Macroalgae act as an excellent form of natural filtration, reducing the available levels of phosphates and nitrites/nitrates. Be sure to thin out the fast-growing Caulerpa regularly; when you remove the clippings, you’re exporting phosphates, nitrates and other nutrients from the tank, thereby helping to maintain good water quality, and periodically harvesting the runners helps keep it from going sexual.
When thinning out or harvesting macroalgae, take care not to actually cut it. Remember, you’re not pruning hedges or trimming trees — the idea is to carefully pull up and remove continuous, unbroken fronds. Simply thin out the colony of excess strands, gently plucking up convenient fronds that can be readily removed intact. A little breakage is fine, but cutting or breaking too many strands will result in leaching undesirable substances into the aquarium water as the Caulerpa’s lifeblood drains away. Too much cutting or breaking can thus sap the colony’s strength and cause die offs or trigger the dreaded vegetative events that judicious pruning otherwise prevents.
Another product I like for removing excess ammonia, nitrite, and nitrate is the Poly-Filter Pad (by Poly-Bio-Marine) Here is a product review on Poly Filters that touches on some good ways to use them:
Click here: Saltwater Aquariums Product Review – Poly-Bio-Marine Inc. – Poly Filter Pad
Speaking of chemical additives, if you use activated carbon in your tank, it’s also very important to make sure that your carbon is phosphate free and that you change it religiously, replacing the old carbon with fresh new carbon every six weeks or so. (If you don’t replace the activated carbon regularly, it could eventually began to leach the wastes and organic compounds it has absorbed back into the aquarium water once it reaches its capacity.) Carbon is activated two ways, either with steam or with phosphoric acid. The type of carbon that is activated with phosphoric acid contains phosphates, which can likewise be leached back into the aquarium water and promote the growth of nuisance algae. So you will want to avoid that type of activated carbon, particularly when you’re having a problem with nuisance algae. The carton or box that the activated carbon came in will be clearly labeled that it is "steam activated" or "phosphate free" or something to that effect if it’s a suitable brand for your aquarium. Activated carbon that is low ash and phosphate-free can help control an outbreak of hair algae if it is changed and replaced with fresh carbon diligently; however, activated carbon that is not free of phosphates or that is not changed regularly can actually contribute to a problem with nuisance algae and degrade your water quality.
Finally, commercially made denitrators (special filters housing a large population of anaerobic denitrifying bacteria) are also available. They do a tremendous job of controlling nitrates but are rather expensive and tend to be high maintenance, often requiring regular "feeding" and carefully controlled flow rates to operate properly.
In summary, some of the measures that will help control nitrates and phosphates (and excess nutrients in general) in the aquarium are the following:
1) Make sure your protein skimmer is working correctly. A protein skimmer works 24 hours a day to remove excess waste and nutrients from a tank. If the venturi is clogged on a venturi skimmer or there is another problem with other skimmer designs, waste will not be exported from your tank and algae will take advantage of the waste.
2) Perform regular water changes. Regular water changes will decrease the level of wastes and nutrients in the water. But the water changes won’t do much good if your tap water itself contains phosphates and amines. Depending on how high the nitrate levels become, increasingthe proportion of water that you change each time may be necessary to help reduce those nitrates. There is an article about nitrate reduction at <<http://www.about.com/>> in the saltwater section that really explains water changes (gives you the math), on actually how little you are reducing nitrates with small water changes when you have high nitrates.
3) Make sure makeup water is pure. Phosphates and nitrates often found in tap water. Phosphate and nitrate test kits will show if your tap water is contributing to your algae problem. If phosphate and nitrate levels are more than 0 ppm (some tap water measures out at over 50 ppm nitrate), filter the water through a RO/DI unit before using it as makeup freshwater or as source water for saltwater changes, or purchase RO water from a vendor.
4) Add additional detritivores to your cleanup crew. If excess food isn’t eaten, it will decay and add to the nutrients and waste in the tank. More microhermit crabs, Nassarius snails and cleaner shrimp will help ferret out any uneaten Mysis before it breaks down and enters the nitrogen cycle to eventually end up as excess nitrate.
5) Introduce macroalgae to consume excess nutrients and nitrates. If regular pruning is done, fast-growing Caulerpa will maintain its color and high growth rates without going sexual. Better yet, an algal filter or "algae scrubber" can be established in a sump or refugium.
6) Chemical controls. Phosphate absorbers can remove excess phosphates just as nitrate sponges will do with nitrates, and Poly Filter pads can also help absorb excess nitrates, changing color as they do so, which helps indicate when the Poly Filter needs to be changed. Low ash activated carbon that is free of phosphates will also help remove such nutrients if it is change religiously and replaced with new carbon.
7) Controlled addition of food to tank. Don’t broadcast feed, scattering Mysis throughout the tank. Instead, target feed your seahorses or use a feeding station. Don’t overfeed, cleanup leftovers promptly, and observe fast days religiously.
8) Add additional live rock and/or a DLSB (preferably in a sump). Both live rock and a deep live sand bed can carry out denitrification and convert nitrate into harmless nitrogen gas, which eventually bubbles out of the aquarium.
Probably the most effective ways to control nitrates in your marine aquarium is to establish an algal filter and a DLSB in a sump. Since your seahorse tank is already equipped with a sump, it would be relatively easy for you to establish an algal filter and install a DLSB in your sump, Tom. That may no if you would be interested in such a project, and I will be happy to provide you with more information explaining how to proceed.
Best of luck getting your nitrate levels back down to 10 ppm or below and easing the breathing of your seahorses, sir. I suspect that the H. reidi are laboring a little bit right now well your H. erectus are thriving is due to the fact that the H. reidi are somewhat more sensitive to high nitrate levels, and this is affecting their breeding. In addition, Mustangs and Sunbursts (Hippocampus erectus) have been captive-bred-and-raised for more generations than H. reidi and are therefore of the hardier and better accustomed to aquarium life. As a result, the erectus may simply shrug off some conditions that your H. reidi find stressful.