Re:frozen mysis

Dear Lawrence:

Wild-caught Brazilians seahorses (Hippocampus reidi) can be notoriously finicky eaters. They are perhaps the most difficult seahorses to wean away from their dependence on live foods. With patience and persistence, most specimens will eventually learn to take high-quality frozen Mysis, but some of the wild reidi can just never make the transition to frozen foods.

Piscine Energetics frozen Mysis relicta may indeed be your best bet for this because individual Mysis thaw out whole and lifelike, with all of their appendages intact, and have natural odor attractants that act as appetite stimulants. The superior PE Mysis relicta is available in smaller quantities online from Premium Aquatics (see link below). The Piscine Energetics frozen Mysis relicta is now available graded for size. You can get the usual jumbo Mysis relicta or smaller Mysis depending on the size of your seahorses.

Click here: Frozen Foods: Premium Aquatics
http://www.premiumaquatics.com/Merchant2/merchant.mvc?Screen=CTGY&Category_Code=Mysis

You can also contact Piscine Energetics and obtain a list of the retail outlets that carry their Mysis relicta. Depending on where you live, you may be able to obtain the PE Mysis relicta from a local fish store in your area:

Click here: Mysis Relicta — Natural fish food,for finicky saltwater and freshwater fish, by Piscine Energetics
http://www.mysis.com/about.html

Yes, sir, as long as you enrich the adult brine shrimp, disinfect it in freshwater before you offer it to your ponies, and your H. reidi will eat it, it’s all right to supplement your seahorse’s diet liberally with the Artemia, as discussed below:

BRINE SHRIMP (Artemia spp.)

Pros (Giwojna, Oct. 1996):
· Adult Artemia are readily available from your fish store or through the mail.
· Easily raised from cysts to provide nauplii of all sizes and stages of development.
· Excellent tolerance for saltwater: feed and forget–survives until eaten.
· Easy to gut-load and enrich.
· Accepted greedily by most seahorses (except Hippocampus reidi and H. ingens).

Cons (Giwojna, Oct. 1996):
· Poor food value–good source of protein, but lacking in other essential nutrients.
· Must be fortified or enriched to increase nutritional content.
· Cannot be used as staple diet.

Collecting Tips:
None

Culture Instructions:
Specific gravity: 1.020-1.026; pH: 8.0-9.0;
Temperature: 77 degrees F (25 degrees C)

An easy way to raise small quantities of brine shrimp is to set up a 10-20 gallon tank in a location where it receives natural sunlight to promote the growth of green algae, and provide gentle aeration using a length of airline tubing as a bubbler (avoid fine bubbles and the use of airstones; Giwojna, Oct. 1996). Sprinkle 1/2 teaspoon of eggs on the surface of the water.

The nauplii will hatch 24-36 hour later, and the day after they emerge, they can be fed sparingly with various additives and enrichment products (Giwojna, Oct. 1996). Adjust the amount so a slight haze barely clouds the water for a few hours each day. Do not feed again until the water is crystal clear, and avoid overfeeding at all costs. Maintain constant aeration to keep the food in suspension, and feed very small amounts fairly often — never a large quantity at any given time (Giwojna, Oct. 1996). The first generation of brine shrimp will reach maturity after 2-3 weeks, and the culture will then be self-sustaining (Daleco Aquarists Supply Manual, 1995). Add more eggs as needed to supplement natural reproduction and bolster the population of brine shrimp. Top off the tank with freshwater regularly to make up for evaporation, and replace about 25% of the culture water on a monthly basis (Giwojna, Oct. 1996).

It’s a good idea to set up 2 or more culture tanks for adult Artemia at the same time so you can harvest a little from each culture and prevent the population of shrimp in any one tank from being depleted to the extent it can no longer sustain itself.

Rearing Artemia this way makes it easy to select nauplii at just the proper stage of development and size for your sea horses (Giwojna, Oct. 1996).

Comments:
Brine shrimp are no doubt the most widely used live foods for sea horses. They are convenient, always available, easy to hatch and raise, and adults can be bought by the pint or quart at many fish stores (Giwojna, Oct. 1996).

However, commercially raised brine shrimp have one big drawback. By the time they are purchased and released in the aquarium, they usually have not eaten for several days, and starved brine shrimp are nutritionally barren. It is therefore imperative that brine shrimp be fortified before they are fed to your sea horses. (As discussed earlier, unfortified adult brine shrimp are useful for feeding to captive-bred seahorses on a staple diet of enriched frozen Mysis on their fasting days precisely because the brine shrimp have nonexistent nutritional value.)

Fortunately, brine shrimp are filter feeders and will take in whatever is suspended in the water with them that has a manageable particle size. This can be yeast cells; unicellular algae; rotifers; micronized rice bran, whey, wheat flour, or egg yolk; dried Spirulina algae; water-soluble vitamin and mineral formulations designed for marine fish; or whatever else the aquarist cares to add to their culture water (Daleco Aquarists Supply Manual, 1995).

I recommend using one of the concentrated food additives or enrichment products that have recently been developed specifically for mariculturists. The best additives are rich in lipids, especially highly unsaturated fatty acids (HUFA), and vitamins such as stabilized Vitamin C and cyanocobalmin (B-12) (Giwojna, Oct. 1996). Adding such enrichment products to a 6-ounce portion of brine shrimp, and then allowing at least 12 hours for the shrimp to ingest it can fortify store-bought adult Artemia (Giwojna, Oct. 1996)

Liquid vitamin formulations can also be added, and the ability to enrich their lipid and vitamin content this way allows us to treat brine shrimp as animated vitamin pills for seahorses (Lawrence, 1998). The savvy seahorse keeper should regard enriched Artemia as bio-encapsulated food for his charges and take full advantage of every opportunity to fortify the shrimp (Lawrence, 1998).

The survival rate of marine fish fry improves dramatically when they are fed lipid-enriched brine shrimp nauplii, and the importance of fortifying Artemia in this manner cannot be overemphasized (Forrest Young, pers. com.). In fact, the Steinhart Aquarium in San Francisco has successfully raised Hippocampus erectus from birth to maturity on a diet consisting solely of brine shrimp (Herald and Rakowicz, 1951). For best results, however, brine shrimp should be considered only a dietary supplement, with of the bulk of your sea horses’ diet consisting of hard-bodied crustaceans such as Mysids, feeder shrimp or Gammarids.

However, Lawrence, if you’ll be supplementing your seahorse’s diet with adult brine shrimp regularly, there are a few precautions you must be careful to observe. First and foremost, you must be careful to disinfect live food beforehand to assure you won’t be introducing any pathogens or parasites along with the prey items, as discussed below.

There is one potentially serious drawback to feeding your seahorses living prey on a regular basis. There is always the chance that you can introduce disease into your aquarium along the with the live food. Live Artemia (brine shrimp), for example, are known disease vectors for a long laundry list of fish pathogens, and should be treated with caution in that regard – especially if obtained from your local fish store (LFS). The aquarist who relies on live foods for his seahorses MUST take special precautions to eliminate this potential danger!

Fortunately, there are a couple of simple measures that can minimize such risks. Decapsulating Artemia cysts, for instance, removes all known parasites and pathogens, effectively sterilizing brine shrimp eggs. Large public aquaria routinely go a step further, disinfecting live foods by administering a 10-minute freshwater bath and then rinsing it thoroughly through a 100-micron strainer before offering it to their seahorses (Bull and Mitchell 2002). Home hobbyists should do the same (a brine shrimp net will suffice for the strainer). Brine shrimp — the chief offender as a disease vector — tolerate this disinfection process extremely well. In addition, adult brine shrimp (Artemia sp.) are now available from High-Health facilities, which greatly minimizes the risk of disease contamination, and if possible, Sandy, you should take full advantage of these safe vendors when purchasing live foods.

Secondly, you must be aware that unfed adult brine shrimp are virtually nutritionally barren, and it is therefore vital that they be enriched improperly before you offer them to your seahorses. It is a great idea to enrich the brine shrimp with Vibrance, but make sure you use the lipid-rich Vibrance I rather than the low-fat formula (Vibrance II) for the Artemia. Adult brine shrimp are a good source of protein, but they have very little fat content. The lipid-rich formulation in Vibrance 1 (the original Vibrance) is thus ideal for enriching brine shrimp, transforming them from nutritionally barren, empty calories into a high-fat powerhouse of vitamins and nutrients that’s loaded with color-enhancing carotenoids. As an added benefit, enriching brine shrimp with Vibrance is also an excellent way to get your seahorses to ingest beta-glucan, which will boost their immune systems and help keep them healthy.

Finally, Lawrence, you need to be aware that adult brine shrimp are not suitable as the staple, everyday diet for your seahorses. In the long run, seahorses that are fed a strict diet of adult brine shrimp will eventually developed a debilitating condition known as soft plate disease:

Soft Plate Disease:

Seahorses and pipefish that receive a diet deficient in calcium are prone to "soft plate" syndrome, which is a progressive disease characterized by decalcification of the bony plates that fuse together to form the exoskeleton (Greco, 2004). In the olden days, seahorses fed a diet consisting solely of Artemia often developed this condition (Greco, 2004). We now know that brine shrimp (Artemia sp.) contains inadequate levels of calcium and an imbalanced ratio of calcium to phosphorus, making it unsuitable as a staple diet even when enriched (Greco, 2004).

Seahorses afflicted with soft plate syndrome exhibit shortened lifespans, decalcification of their exoskeleton, and poor survival rate amongst their fry (Greco, 2004). Pregnant males face the greatest risk of soft plate. Seahorse fry are known to incorporate calcium provided by their father into their skeletons during their embryonic development, so when a gravid male is deficient in calcium, his rapidly growing offspring typically suffer high mortalities due to a condition akin to rickets in human children.

Fortunately, this debilitating condition is easily prevented by providing seahorses with adequate levels of bioavailable calcium either in their diet or in the aquarium water itself (minerals can be obtained by fish directly from the water; Greco, 2004). I have never heard of a case of soft plate in a seahorse kept in a reef tank that received Kalkwasser (calcium hydroxide) via an automatic doser or regular supplementation of bioavailable calcium. Nor have I even seen this condition in seahorses that received a stable diet of enriched frozen Mysis relicta.

In short, Lawrence, don’t hesitate to supplement your seahorse’s diet freely with liberal feedings of adult brine shrimp as long as you enrich the Artemia with Vibrance I, disinfect it beforehand, and don’t rely on it for their staple diet. If you will be feeding your H. reidi primarily on enriched brine shrimp, be sure to make a concentrated effort to include some hard-bodied crustaceans (volcano shrimp, small ghost shrimp, Gammarus amphipods, etc.) in its diet as often as possible.

Best of luck teaching your stubborn H. reidi to accept frozen Mysis, Lawrence!

Happy Trails!
Pete Giwojna