- This topic has 2 replies, 3 voices, and was last updated 16 years, 11 months ago by carrieincolorado.
November 9, 2006 at 1:39 pm #987nigelseahorseMember
I am so happy. My Pair o\’ mustangs came! They look very healthy. I think the male I received is pregnant! . :woohoo: his pouch looks big. Both are dining on red shrimp. I better get started with BBS. how do you decapsulate brine eggs?November 9, 2006 at 2:26 pm #3024Pete GiwojnaGuest
Congratulations on your new arrivals, sir! It’s good to hear that they are eating already; sounds like they’re making themselves right at home.
Yup, if the male is obviously gravid, it would be a good idea to kick your brine shrimp hatcheries into high gear. As you know, decapping the cysts or eggs will improve the nutritional value of the Artemia nauplii. If you feed newly hatched brine shrimp to seahorses immediately after it hatches, while the yolk supply is still largely intact, it needs no enriching (indeed the 1st instar nauplii lack mouthparts and cannot ingest any enrichment formula). Baby brine shrimp that are several hours old have depleted their yolk and undergone their first molt, soon becoming 2nd-instar nauplii complete with mouthparts; they must be enriched after that point or they are nutritionally barren. The 2nd-instar brine shrimp nauplii, and Artemia at all subsequent stages of development, are filter feeders and will take in whatever is suspended in the water with them, including enrichment products such as Vibrance. Here is some more information on decapsulating, hatching, and enriching brine shrimp you should find helpful:
Hatching Out Brine Shrimp (Artemia)
Many commercially made hatcheries are available or you can easily improvise your own from 2-liter soda pop bottles or quart jars. Fill the jars or bottles about 4/5 full with saltwater or brine solution and equip each container with an airstone connected to a length of rigid airline tubing that reaches all the way to the bottom. An inexpensive vibrator air pump with a set of gang valves with put out enough air for the entire battery of hatching containers. Add 1/8-1/4 teaspoon of brine shrimp eggs to each container and adjust the valves so the airstones bubble vigorously, keeping the eggs in suspension at all times. Shine a light directly on the hatching bottles and keep them illuminated 24 hours a day. A temperature of 80-82 degrees F is optimum for hatching brine shrimp.
The eggs will begin hatching after 1-24 hours, and the emerging nauplii should be harvested and used as soon as possible after incubation while they still retain their full nutritional value. (The yolk supply lasts about 6-8 hours after hatching, and the food value of the nauplii deteriorates steadily as the yolk sac is consumed. Once it has been exhausted after about 8 hours, the nutritional worth of the nauplii drops drastically.)
However, before they can be used as food, the nauplii must first be separated from the indigestible egg shells. Otherwise the empty shells may be accidentally ingested by the seahorse fry, which has been known to cause intestinal blockages and death.
The brine shrimp nauplii can be separated from the eggs simply by turning off the air for a few minutes and allowing the water to settle. The unhatched eggs will sink to the bottom of the hatching jar while the empty egg shells will float to the top. The nauplii can then be concentrated in the center of the jar by darkening the room and shining a flashlight on the jar’s midsection. (Brine shrimp are attracted to light and will be drawn together in midwater where the light is focused.) Harvest the nauplii by using a siphon or turkey baster to suck up the concentrated mass of shrimp. The shrimp-laden water can then be strained through a plankton screen or fine-meshed brine shrimp net.
Return the strained water to the hatching container, add more eggs, and readjust the aeration. The same hatching solution can be used for a week’s worth of hatchings before it has to be replaced.
Alternating the hatching container from which you harvest each day’s supply of nauplii will assure that you have a nonstop supply of newly hatched brine shrimp available at all times.
If you’re still uncertain about how to proceed, the information at the following link should make everything perfectly clear:
Click here: Brine Shrimp Technical Information 1
The best eggs or cysts to use for your brine shrimp factory are decapsulated eggs which have had their hard, outer shells stripped away. These shell-less eggs have many advantages over ordinary Artemia cysts. For starters, they simplify the task of separating the live nauplii from the unhatched eggs, since there are no empty shells, and the decapsulated eggs eliminate the possibility of clogged intestines due to the indigestible cysts. Secondly, the decapsulation process destroys virtually all known pathogenic organisms. Since the shell-less eggs have been disinfected, there is much less risk of introducing disease or parasites to the aquarium when you feed your seahorses with brine shrimp from decapsulated cysts. More importantly, the nauplii produced from decapsulated eggs have greater caloric value than the nauplii from unaltered cysts. This is because the nauplii from decapsulated eggs do not have to waste energy struggling to break free of their shells, and thus emerge with 20% greater food value, primarily in the form of additional amino acids and essential fatty acids. This extra nutritional value can make a crucial difference to the rapidly growing seahorses.
Decapsulated brine shrimp eggs are now available from some manufacturers. Although the shell-less eggs are expensive to buy, it is easy for the serious hobbyist to decapsulate his own brine shrimp eggs at home.
Decapsulating Brine Shrimp Eggs.
Decapsulating brine shrimp cysts — the process of dissolving away their hard outer shell — may sound intimidating at first and may seem awkward when you first attempt it. No doubt you will have these instructions open, your eyes glued to the page, with all of your supplies at the ready the first few times you perform this procedure. Relax, this is not difficult at all, and after you’ve done it a couple of times, you will see how truly easy it is and realize decapping is well worth the extra few steps. I will walk you through each numbered step. Measurements do not have to be exact. Regular strength bleach is best, but ultra bleach can be used at lesser portions. You can estimate this yourself. Decapsulating your cysts is beneficial for a number of reasons:
· Reduces the risk of hydroids.
· Removes the outer shell, which means less mess and no fouling of your tank.
· Eliminates intestinal blockages from accidental ingestion of indigestible shells.
· Kills off any and all unwanted contaminants.
· Slightly quicker hatching times.
· Better hatch rates.
· Increased nutritional value secondary to less energy expenditure during hatching.
Supplies Needed for Decapsulating:
· Brine shrimp net
· Air pump
· plastic clip or paper clip wrapped in baggie to clip airline into the container
· Approximately 2 teaspoons brine cysts.
· Approximately 2/3 cup of bleach
· Approximately 2 cups of water
1. Pour your water into a container and clip airline tubing to the side. (No air stone is needed for this). This will keep the cysts in motion. Allow the cysts to aerate this way for approximately 1 hour or a little more.
2. Add in your bleach and continue aerating. As the outer shell gradually dissolves, the eggs go through a series of color changes from brown to gray to white and finally to orange–the color of the nauplii within. This process takes about 7 minutes. The decapsulation process is complete when your cysts become an orange-yellowish color.
3. Pour decapsulated eggs into a brine shrimp net. Add a dechlorination product if you want and rinse until you no longer smell bleach.
4. Drop eggs into your hatching container. You can also refrigerate eggs for about 1 week prior to use in a supersaturated saline solution.
You will need to either feed the bbs to your seahorses immediately after hatching, when their yolk supply is virtually intact and they have their maximum nutritional value, or feed bbs that are 2-days old or older and have been enriched prior to feeding.
Enriching Brine Shrimp at Advanced Instars
Feeding baby brine shrimp his the key to raising nutritious nauplii for your juvenile seahorses. Newly hatched brine shrimp deplete their yolk supply within 6-8 hours and must be fed regularly thereafter to maintain their food value.
Fortunately, brine shrimp are filter feeders and will take in whatever is suspended in water with them. This makes it easy for the aquarist to load the shrimp he is raising with nutritional value by giving them a healthy diet supplemented with special food additives. Commonly used foods for culturing Artemia include unicellular algae; rotifers; yeast-based emulsions; micronized egg yolk, rice bran, wheat flour or whey; and dried Spirulina algae.
Research has proven that brine shrimp can be further enriched by adding supplements such as cuttlefish liver oil, cod liver oil, corn oil, fat-soluble vitamins, amino acids, and mineral formulations to their culture water. Analysis of the nutritional content of culture animals after they had been exposed to such supplemental additives showed a dramatic increase in long-chain fatty acids and many vitamins.
Rather than experimenting with your own concoctions, I recommend using one or more of the lipid-rich food concentrates which have recently been developed specifically for use in aquaculture. Products commonly used by professional breeders for fortifying brine shrimp nauplii include Beta Meal, amino acid and essential vitamins (liquid multi-vitamins), commercial products of (W3) highly unsaturated fatty acids such as Vibrance 1, Selcon Concentrate, Selco, Culture HUFA, Roti-Rich, Astaxanthene biological pigment Natu-Rose, AlgaMac 2000, MicroMac 70, and unicellular microalgae cultures (e.g., T-iso, T-weiss, and Nannochloropsis, Chlorella and Isochrysis sp.). Such products are typically rich in amino acids, highly unsaturated fatty acids (HUFA) and vitamins, which makes them ideal supplements for culturing Artemia. Very often, using a combination of these enrichment products provides better nutrition and produces better results that relying on any one product alone.
For best results, 24 hours after the culture tanks are seeded with newly hatched brine shrimp, begin feeding the nauplii sparingly by adding a concentrated food supplement or enrichment formula according to the instructions. Adjust the amount so that a slight haze barely clouds the water for a few hours every day. Do not feed again until the water is crystal clear and do not overfeed. As the brine shrimp grow, you may need to adjust the dosage of your favorite enrichment product by either increasing the frequency or the amount of the feedings.
The best way to harvest the enriched nauplii is to use a plankton collector or strain the culture water through a plankton screen (available from Florida Aqua Farms). As your seahorses grow, you can sift the nauplii through plankton screens with progressively larger mesh, selecting only the shrimp that are at just the right stage of development for the size of your juveniles.
Best of luck with your new Mustangs and their first brood of young, Nigel!
Pete GiwojnaNovember 9, 2006 at 3:03 pm #3025carrieincoloradoGuest
Woohoo! So glad your horses arrvied safely! Do you have some pictures? Mine are expected today and I am told I have a pregnant male as well! So I’ll be busy doing the same stuff as you, do share your experiences, ok?
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